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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
13/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, D. A. F. da; COTTA, S. R.; VOLLÚ, R. E.; JURELEVICIUS, D. A. de; MARQUES, J. M.; MARRIEL, I. E.; SELDIN, L. |
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Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
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Título: |
Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 332, p. 1-9, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507. MenosBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed... Mostrar Tudo |
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Palavras-Chave: |
Enzima PAT; Proteína Cry. |
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Thesagro: |
Alimento transgênico; Milho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02526naa a2200241 a 4500
001 2008692
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
100 1 $aFERREIRA, D. A. F. da
245 $aEndophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.$h[electronic resource]
260 $c2014
520 $aBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507.
650 $aAlimento transgênico
650 $aMilho
653 $aEnzima PAT
653 $aProteína Cry
700 1 $aCOTTA, S. R.
700 1 $aVOLLÚ, R. E.
700 1 $aJURELEVICIUS, D. A. de
700 1 $aMARQUES, J. M.
700 1 $aMARRIEL, I. E.
700 1 $aSELDIN, L.
773 $tBMC Microbiology$gv. 14, n. 332, p. 1-9, 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
20/04/2006 |
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Data da última atualização: |
17/07/2017 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ANDOW, D. A.; FONTES, E. M. G.; HILBECK, A.; JOHNSTON, J.; CAPALBO, D. M. F.; NELSON, K. C.; UNDERWOOD, E.; FITT, G. P.; SUJII, E. R.; ARPAIA, S.; BIRCH, A. N. E.; PALLINI, A.; WHEATLEY, R. E. |
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Afiliação: |
D. A. ANDOW, University of Minnesota; ELIANA MARIA GOUVEIA FONTES, CENARGEN; A. HILBECK, Swiss Federal Institute of Technology Zurich; J. JOHNSTON; DEISE MARIA FONTANA CAPALBO, CNPMA; K. C. NELSON, University of Minnesota; E. UNDERWOOD; G. P. FITT; E. R. SUJII; S. ARPAIA, Italian National Agency for New Technologies, Energy and Environment; A. N. E. BIRCH; A. PALLINI; R. E. WHEATLEY, Scottsh Crop Research Institute. |
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Título: |
Supporting risk assessment of Bt cotton in Brazil: synthesis and recommendations. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: HILBECK, A.; ANDOW, D. A. FONTES, E. M. G. Methodologies for assessing Bt cotton in Brazil. In: KAPUSCINSKI, A. R.; SCHEI, P. J. Environmmental risk assessment of genetically modified organisms. WALLINGFORD: CABI PUBLISHING, 2006. v. 2, p. p. 346-361. |
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Idioma: |
Inglês |
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Conteúdo: |
Capítulo 13: Cotton in Brazil and the Lepidopteran pests. Problem formulation and options assessment. Transgene expression and locus structure. Non-target and biodiversity effects. Gene flow and consequences. Resistance risk and management. Broad implications of the case study: the environmental risks associated with Bt cotton in Brazil will vary among the different regions of Brazil. Recommendations and future outlook. References. |
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Palavras-Chave: |
Algodão Bt; Brasil. |
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Thesagro: |
Algodão; Impacto ambiental; Planta transgênica. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/130304/1/2006PL-008.pdf
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Marc: |
LEADER 01502naa a2200325 a 4500
001 1011647
005 2017-07-17
008 2006 bl uuuu u00u1 u #d
100 1 $aANDOW, D. A.
245 $aSupporting risk assessment of Bt cotton in Brazil$bsynthesis and recommendations.
260 $c2006
520 $aCapítulo 13: Cotton in Brazil and the Lepidopteran pests. Problem formulation and options assessment. Transgene expression and locus structure. Non-target and biodiversity effects. Gene flow and consequences. Resistance risk and management. Broad implications of the case study: the environmental risks associated with Bt cotton in Brazil will vary among the different regions of Brazil. Recommendations and future outlook. References.
650 $aAlgodão
650 $aImpacto ambiental
650 $aPlanta transgênica
653 $aAlgodão Bt
653 $aBrasil
700 1 $aFONTES, E. M. G.
700 1 $aHILBECK, A.
700 1 $aJOHNSTON, J.
700 1 $aCAPALBO, D. M. F.
700 1 $aNELSON, K. C.
700 1 $aUNDERWOOD, E.
700 1 $aFITT, G. P.
700 1 $aSUJII, E. R.
700 1 $aARPAIA, S.
700 1 $aBIRCH, A. N. E.
700 1 $aPALLINI, A.
700 1 $aWHEATLEY, R. E.
773 $tIn: HILBECK, A.; ANDOW, D. A. FONTES, E. M. G. Methodologies for assessing Bt cotton in Brazil. In: KAPUSCINSKI, A. R.; SCHEI, P. J. Environmmental risk assessment of genetically modified organisms. WALLINGFORD: CABI PUBLISHING, 2006.$gv. 2, p. p. 346-361.
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